Screening for β-Mannosidases with Transmannosidation Capacity.
نویسندگان
چکیده
Several purification and characterization studics have been reported en enzymes from such sources as pineapple,"} rnushroom,2) hen oviduct,3) snails,barley,S] and from microbial seurces su ¢h as these of alkatophilic Bacillu.s speciese) and Aspergiltus niger,7'S) However, the use ef fimannosidase in transmannosidation studies have not been reported. The enzymatic method for the synthesis of glycosides is now considered
منابع مشابه
Activity of α- and β-mannosidases in semen and reproductive organs of the drake
The activity of αand β-mannosidase (α-MAN and β-MAN) in seminal plasma, spermatozoa and homogenates of reproductive organs has been determined in the drake. The highest specific activities of the examined mannosidases were found in epididymides. The activities of both enzymes decreased significantly during the postbreeding resting season compared to the breeding season. Elution profiles of mult...
متن کاملInsights into the structure and function of fungal β-mannosidases from glycoside hydrolase family 2 based on multiple crystal structures of the Trichoderma harzianum enzyme.
UNLABELLED Hemicellulose is an important part of the plant cell wall biomass, and is relevant to cellulosic ethanol technologies. β-Mannosidases are enzymes capable of cleaving nonreducing residues of β-d-mannose from β-d-mannosides and hemicellulose mannose-containing polysaccharides, such as mannans and galactomannans. β-Mannosidases are distributed between glycoside hydrolase (GH) families 1...
متن کاملCorrection: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
C. canimorsus 5 has the capacity to grow at the expenses of glycan moieties from host cells N-glycoproteins. Here, we show that C. canimorsus 5 also has the capacity to deglycosylate human IgG and we analyze the deglycosylation mechanism. We show that deglycosylation is achieved by a large complex spanning the outer membrane and consisting of the Gpd proteins and sialidase SiaC. GpdD, -G, -E an...
متن کاملCellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems.
Evidence shows that bacteria contribute actively to the decomposition of cellulose and hemicellulose in forest soil; however, their role in this process is still unclear. Here we performed the screening and identification of bacteria showing potential cellulolytic activity from litter and organic soil of a temperate oak forest. The genomes of three cellulolytic isolates previously described as ...
متن کاملEffects of Alpha-Lipoid Acid on Serum Iron, Total Iron Binding Capacity and Hematological Indices in β-Thalassemia Major Patients: A Cross Over Clinical Trial
Background and Objectives: Accumulation of iron is the most common consequences of repeated blood transfusions in β-thalassemia major patients. Iron chelating effects of alpha-lipoic acid have been reported by several studies. The aim of this study was to investigate effects of supplementation with alpha-lipoic acid, as a chelator, on serum iron and hematological indices in these patients. Ma...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 56 5 شماره
صفحات -
تاریخ انتشار 1992